Accelerate your cell research
What are your cells doing over time? And over a long time? How do they develop? Where do they go? And on which itineraries? Do they change shape? And to what degree?
Phase Holographic Imaging provides an innovative tool that lets you continuously image and monitor your cells directly inside your incubator even over long periods of time.
The HoloMonitor® M4 is a small microscope designed to operate 24/7, in a small corner of your incubator. Without any labels or stains, it uses digital holography to record your cells in real-time.
The result is real 3D images and quantitative data on your cultures, all the way down to single-cell level, collected in a completely non-invasive way. And beyond standard data be amazed with all the morphological parameters you can examine this way. Just by looking at cells with a holographic view.
HoloMonitor M4
HoloMonitor is a time-lapse cytometer for label-free in vitro live cell imaging and analysis, directly in your incubator.
HoloMonitor is used by scientists all over the world to study the life cycle and behavior of living - well, or dying - cells, under physiologically optimal conditions. No stains needed.
A non-invasive incubator microscope
The employed technique, holographic microscopy, is cell-friendly, fast and simple to use, offering unique imaging capabilities for live-cell kinetic investigations, at both single-cell and cell-population levels.
The HoloMonitor M4 live cell imaging system is designed to visualize and quantify adherent cells over time. The system software, App Suite, allows researchers to create publication-ready images, videos and diagrams of cellular behavior, using a range of applications - all label-free.
Further Information
One sample - multiple results
The heart of HoloMonitor is the modular App Suite software, with tailor-made apps for different live cell assays. Each app offers a guided setup of your experiment, automatic image collection and real-time data analysis and presentation.
The Applications
• Kinetic Cell Morphology (with 30+ parameters)
• Wound Healing (Scratch assay)
• Cell Growth | Cell Division Cell Cycle
• Cytotoxicity & Cell Death
• In-depth Single Cell Tracking & Analysis
• Chemotaxis
• Cellular Differentiation
• Cell Counter
• Kinetic Cell Motility & Migration
• Kinetic Cell Proliferation
• Kinetic Drug Dose-Response
• Cell Quality Control (QC)
EXAMPLES FROM THE APPSUITE CELL ANALYSIS SOFTWARE
HoloMonitor® M4
- Light source: External laser unit
- Sample illumination: 635 nm, 0.2 mW/cm2
- Objective: 20x
- Lateral resolution: 1 μm
- Field of view: 567 μm × 567 μm
- Working distance: 0.5 – 2 mm
- Autofocusing range: 1.5 mm
- Image capture rate: 1 image/s
- Image size: 1024 × 1024 pixels
- Stage travel range: 100×70×10 (x×y×z)
- Stage repeatability: 5 μm
- HoloMonitor M4® dimensions: 290×200×190 mm (w×d×h)
- Space required in incubator: 400×270×190 mm (w×d×h)
- Weight: 5.15 kg
Computer Requirements
- Operating system: Windows 10, 64–bit
- Processor: Intel Core i7 (8th Gen)
- Memory: 16 GB RAM (min 8 GB)
- Hard drive: 512 GB SSD (min 256 GB)
- Display: Full HD (min 1920×1080)
- Other: 2 built–in USB ports
Sample and Environment
- Cells: Adherent cell monolayer
- Cell culture vessels: 6-, 24-, 96-well, Petri, IBIDI
- Operating temperature: 10-40° C
- Operating humidity: Max 95 %
- For research use only
The holomonitor live cell assays
Cell QC Assay
Evaluate cell integrity and
detect changes of your cell culture.
Dose Response Assay
Recognize drug toxicity patterns and
kinetics of drug effects from interactive
dose response curves.
Single Cell Tracking
Study single cell motility and migration
patterns and distinguish between cell
subpopulation behavior.
Cell Counter
Determine the exact cell
number in your cell suspension.
Cell Motility Assay
Explore average cell population motility
based on cell speed and accumulated
cell distance in real–time.
Kinetic Morphology
Examine more than 30 parameters,
as cell morphology can be sensitive
markers for cell damage.
Cell Proliferation Assay
Robust kinetic cell proliferation
assessment by direct identification and
counting of individual cells.
Wound Healing Assay
Easily obtain kinetic information on how
cell populations close the wound gap and
determine cell front velocity
Culture Vessel Holders
HoloMonitor’s motorized stage supports a variety of culture vessels, each secured with a vessel holder. This means easy attachment and exact positioning on the microscope.
HoloLids™
HoloLids are specially designed lids for standard cell culture vessels. They are optically superior to normal vessel lids and ensure the best possible image quality every time.
HoloDry™
HoloDry is a non-toxic canister that is easily attached to the HoloMonitor microscope. It keeps the optics and interior free of condensation, which ensures optimal performance.